Anonymous Modernist 3603

Joe, while you are correct that there are certain practical force minimums (aka you don't want to spin down for 20k hours), the point that I was trying to illustrate is that force alone is not enough to determineseparationresult - time must also be taken into account. This is mostanalogousto temperatures for foodsafetydiscussedin chapter 2. Temperature alone is of no use, time at that temperature must also be used. There do exist extreme specialcircumstances, mostly in the biological sciences, where you do need a higher force, but those are few and far between. Seattle, and anyone else thinking about one, if I remember correctly, the TJ-6 is a swingbucketcentrifuge (vs a fixed angle). Be EXTREMELY careful with such models (don't mean to scare, just impact a healthyappreciation). As I'm sure you can imagine, should one of those buckets come loose, the result arecatastrophic, and potentially fatal. Tolerances are typically very high, adn defects can go undetectable by human eye; it's important to keep the machine well lubricated and cares for. Further, to achieve proper balance on a swingbucket, all buckets must be balanced witheach other, not just apposing sets - something that was told to me by a Beckman rep.

Theoretically speaking, anyseparationtask can be carried out at any force >0G, given an appropriate time. The greater the force however, the smaller the amount of time necessary. The amount of force supplied by thecentrifugeis a factor of both how fast the rotor spins (machine dependent) as well as the distrance and angle of the sample from the center of rotation (rotor dependent). When selecting a rotor, there is often times a factor that will be inscribed, by which to calculate the RCF (RelativeCentrifugalForce) imparted. Some of the specifications for spin force and time may be somewhatexaggerated (insurepelletforms, increase pellet density, etc), while others may be just enough. As such, the time it requires to spin at a lesser force may or may not need to be increased - you'll likely have toexperimentto see how much of a difference a decrease in force makes. RCF Calculator:https://www.beckmancoulter.com/wsrportal/wsrportal.portal?_nfpb=true&_windowLabel=UCM_RENDERER&_urlType=render&wlpUCM_RENDERER_path=%2Fwsr%2Fresearch-and-discovery%2Fproducts-and-services%2Fcentrifugation%2Frotors%2Findex.htm&wlpUCM_RENDERER_t=3