At one establishment in Albany, NY I asked and was told that the calamari was allowed to 'cure' in the marinade for a very long time and implied that it was for more than 24 hours. If true and factual I have to assume that would be similar to a long simmer in a cooked dish. However, I do not know the biochemistry behind the protein-acid reaction to know. Are there any good research libraries on line where a person could do read up on applicable Food Science?