Hi all, I am rather stupmed at what happened in my circulation bath this week and would like to share and maybe get to the bottom of what exactly might have caused this. Quick background: I am a German chef having learned at a 2* Michelin restaurant and now CEO of a burger chain in Germany. I have a passion for biochemistry and approach virtually all cooking endeavours with an analytic mindset. As I prepared a lasagna based on braised oxtail last weekend I was left with about 1300g of oxtail chunks that didn't fit into my pressure cooker. Whilst braising I browsed my copy of MC searching for an interesting idea for said left over produce and stumbled across the 100 h @60C preparation. I scaled the recipe to the required amount of stock and boiled it up quickly as to reduce any possible pathogens. I then filled the sous vide bag with the raw, unseasoned and chilled (4C) oxtail, added the cooled stock (13C) and sealed in a chamber vaccum to -1 bar. I then introduced the bag into the water bath at 60.0C, sealed the bath with cling film and checked througout the following days. For the duration of the cook the bag stayed at the bottom of the bath and temperature fluctuations were at a maxiumum of 0,1C. Yesterday morning (thursday, I started the cook on sunday at around lunchtime) I saw that a small bubble of bombage had formed over night. Weary as to the cause, I was even more suprised to find the entire bag bloated and floating at the top of the surface when I came home last night. Unsuprisingly the smell after opeing the bag was horrible and had obviously spoiled. I am asbolutely flabbergasted as to what might possibly have caused this. Shouldn't any possible pathogen have been killed of after at least 80h @ 60C with no sign of bacterial activity? A couple of chefs I work with are equally as stumped as I and I would love, if someone on this brilliant forum could possibly offer some insight. So thanks for your feedback which I would love to incorporate into the next try; after all: failure is not an option! ;-) All the Best, Johannes